Peptide Dosage
5mg vial, 2mL BAC water, 250mcg dose
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Evidence-based calculations Used in clinical settings worldwide Regular monitoring recommended
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💊 BPC-157 (5mg vial)
5mg vial, 2mL BAC water, 250mcg dose
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💉 Semaglutide (2mg vial)
2mg vial, 1mL diluent, 0.25mg starting dose
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🏋️ HGH (10IU vial)
10IU vial, 1mL BAC water, 2IU dose
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💊 Tirzepatide (5mg vial)
5mg vial, 2mL diluent, 2.5mg dose
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🧬 TB-500 (5mg vial)
5mg vial, 2mL BAC water, 2mg loading dose
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Common Peptides
💊 BPC-157 (5mg vial)
5mg vial, 2mL BAC water, 250mcg dose
💉 Semaglutide (2mg vial)
2mg vial, 1mL diluent, 0.25mg starting dose
🏋️ HGH (10IU vial)
10IU vial, 1mL BAC water, 2IU dose
💊 Tirzepatide (5mg vial)
5mg vial, 2mL diluent, 2.5mg dose
🧬 TB-500 (5mg vial)
5mg vial, 2mL BAC water, 2mg loading dose
Enter Parameters
Dosing Results
Concentration
mcg/mL
Injection Volume
Syringe Units
(10 tick marks)
Doses per Vial
(20 days supply)
💡 Tips
Store reconstituted peptide in refrigerator
Use reconstituted peptide within 4-8 weeks
Avoid shaking - gently roll to mix
Disclaimer: This calculator is for informational purposes only. Consult a healthcare provider before using any peptides. Peptides may not be approved for human use in some jurisdictions.
For informational purposes only — not medical advice. Consult a healthcare professional before acting on results.
🏥 Health Facts
— WHO
— CDC
What is Peptide Reconstitution?
Peptides are small proteins typically sold as lyophilized (freeze-dried) powder that must be reconstituted with bacteriostatic water (BAC water) before use. The reconstitution process determines the final concentration, which is essential for accurate dosing. This calculator helps you determine exactly how much solution to draw for your desired dose using standard insulin syringes.
Reconstitution
Dissolving lyophilized peptide powder with BAC water to create an injectable solution.
Key Steps:
- Add water slowly along vial wall
- Gently roll - never shake
- Wait for powder to dissolve
Proper Storage
Reconstituted peptides are delicate and require careful storage to maintain potency.
Storage Rules:
- Refrigerate at 2-8°C (36-46°F)
- Use within 4-8 weeks
- Protect from light
Insulin Syringes
Standard insulin syringes are used for precise peptide dosing with unit-based measurement.
Syringe Types:
- U-100: 100 units = 1 mL
- U-50: 50 units = 0.5 mL
- U-30: 30 units = 0.3 mL
How Does This Calculator Work?
This calculator converts your vial strength and desired dose into exact syringe units. More BAC water = lower concentration = more volume per dose. Less water = higher concentration = less volume per dose. The math can be confusing, but this tool makes it simple.
🔬 Calculation Process
Step-by-Step
- 1Enter vial strength (e.g., 5mg peptide)
- 2Enter BAC water volume added (e.g., 2mL)
- 3Enter desired dose per injection
- 4Get exact syringe units to draw
Why Volume Matters
- More water = easier to measure small doses
- Less water = fewer injections per vial
- Standard: 1-2mL per 5mg vial is common
- Accuracy improves with larger injection volumes
When to Use This Calculator
This calculator is essential for anyone using research peptides or prescribed peptide medications that require reconstitution. Accurate dosing is critical for safety and effectiveness.
Research Peptides
BPC-157, TB-500, and similar research peptides sold in lyophilized form.
Common Uses:
- BPC-157: 250-500 mcg
- TB-500: 2-5 mg loading
- CJC-1295: 100-300 mcg
GLP-1 Medications
Semaglutide, tirzepatide, and similar weight loss peptides from compounding pharmacies.
Typical Doses:
- Semaglutide: 0.25-2.4 mg/week
- Tirzepatide: 2.5-15 mg/week
Growth Hormone
HGH measured in IU (International Units) requires different calculation approach.
HGH Dosing:
- Anti-aging: 1-2 IU/day
- Performance: 2-4 IU/day
- 10IU vial = 3.3mg
Peptide Dosing Formulas
Understanding these formulas helps you verify calculations and adapt to different scenarios. The key is converting between mass units (mg, mcg) and volume (mL, syringe units).
📊 Core Formulas
Step 1: Calculate Concentration
Example: 5000mcg ÷ 2mL = 2500 mcg/mL
Convert mg to mcg by multiplying by 1000
Step 2: Calculate Injection Volume
Example: 250mcg ÷ 2500mcg/mL = 0.1 mL
This is how much solution to draw
Step 3: Convert to Syringe Units
Example: 0.1 mL × 100 = 10 units
Draw to the "10" mark on U-100 insulin syringe
Doses per Vial
Example: 5000mcg ÷ 250mcg = 20 doses
Plan your vial usage accordingly
Common Peptides and Typical Dosing
Different peptides have varying typical doses and administration frequencies. Always verify with your healthcare provider.
BPC-157
Healing and recovery peptide
Typical dose: 250-500 mcg
Frequency: 1-2x daily
Vial size: 5mg typical
Route: SubQ near injury site
Semaglutide
GLP-1 for weight management
Start: 0.25mg weekly × 4 weeks
Titrate: Increase every 4 weeks
Maintenance: 1.0-2.4mg weekly
Route: SubQ (abdomen/thigh)
Tirzepatide
Dual GIP/GLP-1 agonist
Start: 2.5mg weekly × 4 weeks
Titrate: 2.5mg increase every 4 weeks
Max: 15mg weekly
Route: SubQ weekly
TB-500
Thymosin Beta-4 fragment
Loading: 5-10mg/week × 4-6 weeks
Maintenance: 2-5mg/week
Frequency: 2-3x weekly
Route: SubQ or IM
CJC-1295/Ipamorelin
Growth hormone secretagogue combo
CJC-1295: 100-200 mcg
Ipamorelin: 100-200 mcg
Frequency: 1-3x daily
Best time: Before bed
Melanotan II
Tanning peptide
Loading: 250-500 mcg daily
Maintenance: 1-2x weekly
Vial size: 10mg typical
Route: SubQ
Step-by-Step Reconstitution Guide
Proper reconstitution technique is essential for peptide stability and accurate dosing. Follow these steps carefully.
Reconstitution Steps
- 1Remove peptide vial from refrigerator, allow to reach room temperature
- 2Clean vial tops with alcohol swab
- 3Draw desired amount of bacteriostatic water into syringe
- 4Inject water slowly against vial wall (not directly onto powder)
- 5Gently swirl vial - NEVER shake
- 6Store reconstituted peptide in refrigerator
Important Tips
- Use bacteriostatic water (contains 0.9% benzyl alcohol)
- Reconstituted peptides last 28-30 days refrigerated
- Never freeze reconstituted peptides
- Keep unreconstituted vials frozen for long-term storage
- Label vials with date reconstituted and concentration
- Never use if solution is cloudy or discolored
Syringe Types and Measurements
Understanding syringe markings is crucial for accurate dosing. Most peptide users use insulin syringes.
U-100 Insulin Syringe
- • 100 units = 1 mL
- • Most common for peptides
- • Available in 0.3mL, 0.5mL, 1mL sizes
- • Fine gauge (29-31G) needles
- • Each unit mark = 0.01 mL
U-40 Insulin Syringe
- • 40 units = 1 mL
- • Less common, for pet insulin
- • DO NOT use for U-100 calculations
- • Different scale markings
Standard (mL) Syringes
- • Marked in mL, not units
- • Larger volumes (1mL, 3mL, etc.)
- • Good for reconstitution
- • Larger needles, less precise
Clinical Pearls
Air Bubbles
Small air bubbles in SubQ injections are harmless but can affect dose accuracy. Tap syringe to move bubbles to top.
Injection Sites
Rotate injection sites to prevent lipodystrophy. Common sites: abdomen, thighs, upper arms.
Pinch Technique
Pinch skin for SubQ injection at 45° angle. Release pinch before withdrawing needle.
Storage Matters
Peptide potency degrades with heat and light. Keep refrigerated and protect from sunlight.
Never Reuse Needles
Use new sterile needle for each injection. Reusing causes infection risk and needle dulling.
Double-Check Math
Always verify your calculations. A small error in concentration can lead to significant dosing errors.
Safety Considerations
Peptide therapy requires careful attention to safety, sterility, and proper handling to avoid complications.
Warning Signs to Stop
- Signs of infection at injection site (redness, swelling, warmth, pus)
- Systemic allergic reactions (hives, difficulty breathing)
- Severe nausea/vomiting (especially GLP-1 agonists)
- Cloudy or discolored solution
Best Practices
- Work under medical supervision when possible
- Source peptides from reputable suppliers with testing
- Maintain sterile technique throughout
- Start with lower doses and titrate up
Common Reconstitution Reference
| Vial Size | Water Added | Concentration | 250mcg Dose |
|---|---|---|---|
| 5mg | 1mL | 5000 mcg/mL | 5 units |
| 5mg | 2mL | 2500 mcg/mL | 10 units |
| 10mg | 2mL | 5000 mcg/mL | 5 units |
| 10mg | 4mL | 2500 mcg/mL | 10 units |
| 15mg | 3mL | 5000 mcg/mL | 5 units |
Units refer to U-100 insulin syringe markings. 1 unit = 0.01mL
Frequently Asked Questions
Why use bacteriostatic water instead of sterile water?
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, allowing the reconstituted peptide to last up to 28 days refrigerated. Sterile water has no preservative and must be used within 24 hours.
Can I use a regular syringe instead of insulin syringe?
Yes, but insulin syringes are preferred for their fine gauge needles (less painful) and precise markings. Regular mL syringes work but may be harder to measure small volumes accurately.
Why shouldn't I shake the vial?
Shaking can denature the peptide, destroying its structure and effectiveness. Peptides are delicate proteins. Always swirl gently to dissolve.
How do I know if my peptide has degraded?
Signs of degradation include cloudiness, particles, discoloration, or unusual odor. If any of these are present, discard the vial. Loss of efficacy without visible signs may also indicate degradation.
What's the difference between SubQ and IM injection for peptides?
Subcutaneous (SubQ) injection is under the skin into fat tissue and is preferred for most peptides due to slower, steadier absorption. Intramuscular (IM) provides faster absorption but may be more painful and has higher infection risk.
Can I mix BPC-157 and TB-500 in the same vial?
While some users combine them for synergistic effects, this isn't well-studied. It's safer to reconstitute separately and inject at different sites. This also allows for individual dose adjustments.
How long do peptide results take?
It varies by peptide and indication. BPC-157 may show benefits within 1-2 weeks for injury healing. GLP-1 agonists like semaglutide typically show weight loss within 4-8 weeks. Growth hormone secretagogues may take 3-6 months for body composition changes.
Storage and Handling Guide
Proper storage is crucial for maintaining peptide potency and safety.
Unreconstituted
- • Store in freezer for long-term
- • Refrigerator for short-term (weeks)
- • Protect from light
- • Keep in original packaging
- • Stable for 1-2 years frozen
Reconstituted
- • Must refrigerate (36-46°F)
- • Use within 28-30 days
- • Never freeze after reconstitution
- • Label with date and concentration
- • Keep in dark container if possible
Signs of Degradation
- • Cloudiness or particulates
- • Color change
- • Unusual odor
- • Clumping of powder
- • Reduced effectiveness
Common Side Effects by Peptide Type
GLP-1 Agonists (Semaglutide, Tirzepatide)
- • Nausea (most common, usually improves)
- • Constipation or diarrhea
- • Injection site reactions
- • Rare: pancreatitis (seek care for severe abdominal pain)
Growth Hormone Peptides
- • Water retention, joint pain
- • Carpal tunnel symptoms
- • Increased hunger (GHRPs)
- • Mild tingling or numbness
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